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SPP1 Antibody

  • WB - SPP1 Antibody ASC11685
    Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - SPP1 Antibody ASC11685
    Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 µg/mL.
  • IF - SPP1 Antibody ASC11685
    Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10451
Other Accession NP_001238759, 352962176
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 36 kDa

Observed: 37 kDa
Application Notes SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 µg/mL.
Additional Information
Gene ID 6696
Target/Specificity SPP1; SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Reconstitution & Storage SPP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsSPP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted.
Tissue Location Bone. Found in plasma.
Research Areas
Citations (0)

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SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).


Heinegard D, Andersson G, and Reinholt FP. Roles of osteopontin in bone remodeling. Ann. N.Y. Acad. Sci.1995; 760:213-22.
Wang KX and Denhardt DT. Osteopontin: rule in immune regulation and stress responses. Cyto. Growth Factor Rev. 2008; 19:333-45.
Boskey AL, Maresca M, Ullrich W, et al. Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel. Bone Miner. 1993; 22:147-159.
Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc. Natl. Acad. Sci. USA 1992; 89:426-30.

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$ 335.00
Cat# ASC11685
Availability: 5-7days
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