|Application ||WB, E|
|Other Accession||NP_079223, 148612879|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 109 kDa |
Observed: 120 kDa
|Application Notes||PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||PHC3; PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.|
|Reconstitution & Storage||PHC3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PHC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.|
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Provided below are standard protocols that you may find useful for product applications.
Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1). PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2). PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3). PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4).
Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409–18.
Deshpande AM, Akunowicz JD, Reveles XT, et al. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors. Oncogene 2007; 26:1714-22.
Isono K, Fujimura Y, Shinga J, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol. Cell Biol. 2005; 25:6694-706.
Iwata S, Takenobu H, Kageyama H, et al. Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma. Cancer Sci. 2010; 101:1646-52.
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