|Application ||WB, IF, ICC, E|
|Other Accession||NP_056066, 54792092|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 73, 79, 91 kDa |
Observed: 72 kDa
|Application Notes||ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry at 5 µg/mL. For Immunoflorescence start at 2.5 µg/mL.|
|Target/Specificity||ATMIN; ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.|
|Reconstitution & Storage||ATMIN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ATMIN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription.|
|Cellular Location||Nucleus. Note=Nuclear, in discrete foci during G1 phase|
|Tissue Location||Ubiquitously expressed in normal tissues and cancer cell lines with highest levels in placenta and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57.
Jurado S, Smyth I, van Denderen B, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010; 6:e1001170.
Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interating Zn2+ finger (ASCIZ) is a bi-functional transcriptional activator and feedback sensor in the regulation of Dynein Light Chain (DYNLL1) expression. J. Biol. Chem. 2012; 287:3156-64.
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