|Application ||WB, E|
|Other Accession||64762489, NP_776250, 169026|
|Calculated MW||40755 Da|
|Application Notes||SLC30A8 antibody can be used for Western blot at 1 - 2 µg/mL.|
|Other Names||SLC30A8 Antibody: Solute carrier family 30 member 8, zinc transporter 8, ZNT8, Zn-T8|
|Precautions||SLC30A8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Note=Associated with insulin and glucagon secretory granules.|
|Tissue Location||In the endocrine pancreas, expressed in insulin-producing beta cells. Expressed at relatively high levels in subcutaneous fat tissue from lean persons; much lower levels in visceral fat, whether from lean or obese individuals, and in subcutaneous fat tissue from obese individuals. Expressed in peripheral blood mononuclear cells, including T-cells and B-cells, with great variation among individuals ranging from negative to strongly positive.|
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Provided below are standard protocols that you may find useful for product applications.
SLC30A8 Antibody: SLC30A8 is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles and is expressed at a high level in the pancreas, particularly in islets of Langerhans, and co-localizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells (1). Allelic variants of SLC30A8 have been identified as major genetic risk factors for the development of Type 2 diabetes (2), but no genetic association has been found with Type 1 diabetes (3). SLC30A8 expression in -cells was found to be influenced by cytokine expression, particularly IFN-gamma and IL-1beta (4).
Chimienti F, Devergnas S, Favier A, et al. Identification and cloning of a beta-cell-specific zinc transporter, ZnT-8, localized into insulin secretory granules. Diabetes 2004; 53:2330-7.;Sladek R, Rocheleau G, Rung J, et al. A genome-wide association study indentifies novel risk loci for type 2 diabetes. Science 2007; 445:881-5.;Qu HQ, Grant SF, Bradfield JP, et al. Association analysis of type 2 diabetes Loci in type 1 diabetes. Diabetes 2008; 57:1983-6.;Egefjord L, Jensen JL, Bang-Berthelsen CH, et al. Zinc transporter gene expression is regulated by pro-inflammatory cytokines: a potential role for zinc transporters in beta-cell apoptosis? BMC Endocrine Disorders 2009; 9:7.
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