|Description||Anti-HUMAN IgG F(ab')2 (RABBIT) Antibody (Min X MOUSE Serum Proteins)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:350,000|
Western Blot 1:3,000-1:15,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG F(ab')2|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Human IgG F(ab')2 antibody detects human IgG F(ab')2 fragments. This secondary antibody anti-Human IgG F(ab')2 is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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