Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Donkey Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||Anti-RABBIT IgG (H&L) (DONKEY) Antibody Alkaline Phosphatase Conjugated|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:25,000|
Western Blot 1:500-1:2,500
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Rabbit IgG whole molecule|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.1% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Anti-RABBIT IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum.|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Anti-Rabbit IgG generated in Donkey detects Rabbit IgG heavy and light chains. This Anti Rabbit antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.