Anti-Rat IgM (mu chain) (Rhodamine Conjugated) Secondary Antibody
Goat Polyclonal, Rhodamine (TRITC)
|Description||Anti-RAT IgM (mu chain) (GOAT) Antibody Rhodamine Conjugated|
|FP Value||2.3 moles Rhodamine (TRITC) per mole of IgG|
|Application ||IF, FC|
|Application Note||FLISA 1:10,000-1:50,000|
IF Microscopy 1:1,000-1:5,000
|Target Isotype||IgM µ chain|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Rat IgM mu heavy chain|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG or Rat IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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