Anti-Rabbit IgG (H&L) Pre-Adsorbed Secondary Antibody
Rat Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||Anti-RABBIT IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins)|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:1,500-1:6,000|
Western Blot 1:500-1:2,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Rabbit IgG whole molecule|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against the sources listed above.|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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