Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (ATTO 532 Conjugated) Secondary Antibody
Rabbit Polyclonal, ATTO 532
|Description||Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody ATTO 532 Conjugated|
|FP Value||2.5 moles ATTO 532 per mole of IgG|
|Application ||WB, IF|
|Application Note||FLISA >1:20,000|
IF Microscopy >1:5,000
Western Blot >1:10,000
|Target Isotype||IgG (gamma 1, 2a, 2b and 3 chain)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins|
|Reconstitution Volume||500 µL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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