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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   PCAF Antibody (monoclonal) (M04)   

PCAF Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant PCAF.

  • WB - PCAF Antibody (monoclonal) (M04) AT3206a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - PCAF Antibody (monoclonal) (M04) AT3206a
    PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in human uterus myoma.
  • WB - PCAF Antibody (monoclonal) (M04) AT3206a
    PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in LNCaP ( (Cat # AT3206a )
  • WB - PCAF Antibody (monoclonal) (M04) AT3206a
    PCAF monoclonal antibody (M04), clone 5E11 Western Blot analysis of PCAF expression in Hela S3 NE ( (Cat # AT3206a )
  • WB - PCAF Antibody (monoclonal) (M04) AT3206a
    PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in A-431 ( (Cat # AT3206a )
  • IF - PCAF Antibody (monoclonal) (M04) AT3206a
    Immunofluorescence of monoclonal antibody to PCAF on HeLa cell. [antibody concentration 10 ug/ml]
  • E - PCAF Antibody (monoclonal) (M04) AT3206a
    Detection limit for recombinant GST tagged PCAF is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92831
Other Accession BC060823
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5E12
Calculated MW 93013 Da
Additional Information
Gene ID 8850
Other Names Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Histone acetylase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, P/CAF, KAT2B, PCAF
Target/Specificity PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPCAF Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Acetylation of lysine 564 adjacent to the C-terminal binding protein-binding motif in EVI1 is crucial for transcriptional activation of GATA2. Shimahara A, et al. J Biol Chem, 2010 May 28. PMID 20363750.CARM1 activates myogenin gene via PCAF in the early differentiation of TPA-induced rhabdomyosarcoma-derived cells. Gao X, et al. J Cell Biochem, 2010 May. PMID 20213728.Regulation of Treg functionality by acetylation-mediated Foxp3 protein stabilization. van Loosdregt J, et al. Blood, 2010 Feb 4. PMID 19996091.Single nucleotide polymorphisms in miRNA binding sites and miRNA genes as breast/ovarian cancer risk modifiers in Jewish high-risk women. Kontorovich T, et al. Int J Cancer, 2010 Aug 1. PMID 19950226.

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$ 350.00
Cat# AT3206a
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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