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PITX1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PITX1.

  • WB - PITX1 Antibody (monoclonal) (M01) AT3319a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
  • WB - PITX1 Antibody (monoclonal) (M01) AT3319a
    PITX1 monoclonal antibody (M01), clone 5G4 Western Blot analysis of PITX1 expression in Hela S3 NE ( (Cat # AT3319a )
  • WB - PITX1 Antibody (monoclonal) (M01) AT3319a
    Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.

    Lane 1: PITX1 transfected lysate(34.1 KDa).
    Lane 2: Non-transfected lysate.
  • IP - PITX1 Antibody (monoclonal) (M01) AT3319a
    Immunoprecipitation of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PITX1 MaxPab rabbit polyclonal antibody.
  • E - PITX1 Antibody (monoclonal) (M01) AT3319a
    Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.
  • WB - PITX1 Antibody (monoclonal) (M01) AT3319a
    Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi ( (Cat # AT3319a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78337
Other Accession NM_002653
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5G4
Calculated MW 34128 Da
Additional Information
Gene ID 5307
Other Names Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1, PITX1, BFT, PTX1
Target/Specificity PITX1 (NP_002644, 225 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPITX1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.


1.The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage.Yamaguchi T, Miki Y, Yoshida K.Apoptosis. 2010 Aug;15(8):927-35.2.Transcriptional activation of p53 by Pitx1.Liu DX, Lobie PE.Cell Death Differ. 2007 Nov;14(11):1893-907. Epub 2007 Aug 31.

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$ 350.00
Cat# AT3319a
Availability: 7-10 days
Bulk Size
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