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PPM1B Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PPM1B.

  • WB - PPM1B Antibody (monoclonal) (M01) AT3406a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (46.86 KDa) .
  • WB - PPM1B Antibody (monoclonal) (M01) AT3406a
    Western Blot analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.

    Lane 1: PPM1B transfected lysate(52.643 KDa).
    Lane 2: Non-transfected lysate.
  • IF - PPM1B Antibody (monoclonal) (M01) AT3406a
    Immunofluorescence of monoclonal antibody to PPM1B on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - PPM1B Antibody (monoclonal) (M01) AT3406a
    Immunoprecipitation of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPM1B monoclonal antibody.
  • E - PPM1B Antibody (monoclonal) (M01) AT3406a
    Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.
  • WB - PPM1B Antibody (monoclonal) (M01) AT3406a
    Western blot analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi ( (Cat # AT3406a )
  •  - PPM1B Antibody (monoclonal) (M01) AT3406a
    Proximity Ligation Analysis of protein-protein interactions between ARRB2 and PPM1B HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-PPM1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75688
Other Accession BC012002
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1A3-2A4
Calculated MW 52643 Da
Additional Information
Gene ID 5495
Other Names Protein phosphatase 1B, Protein phosphatase 2C isoform beta, PP2C-beta, PPM1B, PP2CB
Target/Specificity PPM1B (AAH12002, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPPM1B Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation. Sun W, et al. Cell Signal, 2009 Jan. PMID 18930133.Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 T-loop. Wang Y, et al. J Biol Chem, 2008 Nov 28. PMID 18829461.Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Xiao K, et al. Proc Natl Acad Sci U S A, 2007 Jul 17. PMID 17620599.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 350.00
Cat# AT3406a
Availability: 7-10 days
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