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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SMURF1 Antibody (monoclonal) (M01)   

SMURF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SMURF1.

  • WB - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.18 KDa) .
  • WB - SMURF1 Antibody (monoclonal) (M01) AT3966a
    SMURF1 monoclonal antibody (M01), clone 1D7. Western Blot analysis of SMURF1 expression in HeLa.
  • WB - SMURF1 Antibody (monoclonal) (M01) AT3966a
    SMURF1 monoclonal antibody (M01), clone 1D7. Western Blot analysis of SMURF1 expression in HepG2.
  • WB - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.

    Lane 1: SMURF1 transfected lysate(83.4 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2 ug/ml]
  • IF - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SMURF1 MaxPab rabbit polyclonal antibody.
  • E - SMURF1 Antibody (monoclonal) (M01) AT3966a
    Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HCE7
Other Accession NM_020429
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D7
Calculated MW 86114 Da
Additional Information
Gene ID 57154
Other Names E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625
Target/Specificity SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSMURF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.


1.Impaired phosphorylation and ubiquitination by P70 S6 kinase (P70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promotes Tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun FJ Biol Chem. 2013 Oct 2.2.Smurf1 ubiquitin ligase causes downregulation of BMP receptors and is induced in monocrotaline and hypoxia models of pulmonary arterial hypertension.Murakami K, Mathew R, Huang J, Farahani R, Peng H, Olson SC, Etlinger JD.Exp Biol Med (Maywood). 2010 Jul 1;235(7):805-13.

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$ 350.00
Cat# AT3966a
Availability: 7-10 days
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