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TGIF2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TGIF2.

  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.4 KDa) .
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    TGIF2 monoclonal antibody (M01), clone 4C10. Western Blot analysis of TGIF2 expression in IMR-32 ( (Cat # AT4228a )
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    TGIF2 monoclonal antibody (M01), clone 4C10. Western Blot analysis of TGIF2 expression in SW-13 ( (Cat # AT4228a )
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    TGIF2 monoclonal antibody (M01), clone 4C10 Western Blot analysis of TGIF2 expression in Hela S3 NE ( (Cat # AT4228a )
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    TGIF2 monoclonal antibody (M01), clone 4C10. Western Blot analysis of TGIF2 expression in Y-79 ( (Cat # AT4228a )
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    TGIF2 monoclonal antibody (M01), clone 4C10. Western Blot analysis of TGIF2 expression in A-431 ( (Cat # AT4228a )
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    Western Blot analysis of TGIF2 expression in transfected 293T cell line by TGIF2 monoclonal antibody (M01), clone 4C10.

    Lane 1: TGIF2 transfected lysate(25.9 KDa).
    Lane 2: Non-transfected lysate.
  • E - TGIF2 Antibody (monoclonal) (M01) AT4228a
    Detection limit for recombinant GST tagged TGIF2 is approximately 0.3ng/ml as a capture antibody.
  • WB - TGIF2 Antibody (monoclonal) (M01) AT4228a
    Western blot analysis of TGIF2 over-expressed 293 cell line, cotransfected with TGIF2 Validated Chimera RNAi ( Cat # H00060436-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TGIF2 monoclonal antibody (M01), clone 4C10 (Cat # AT4228a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZN2
Other Accession NM_021809
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4C10
Calculated MW 25878 Da
Additional Information
Gene ID 60436
Other Names Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, TGF-beta-induced transcription factor 2, TGFB-induced factor 2, TGIF2
Target/Specificity TGIF2 (NP_068581, 131 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTGIF2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is a DNA-binding homeobox protein and a transcriptional repressor. The encoded protein appears to repress transcription by recruiting histone deacetylases to TGF beta-responsive genes. This gene is amplified and overexpressed in some ovarian cancers, and mutations in this gene can cause holoprosencephaly.


Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Differentially regulated genes as putative targets of amplifications at 20q in ovarian cancers. Watanabe T, et al. Jpn J Cancer Res, 2002 Oct. PMID 12417041.

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$ 350.00
Cat# AT4228a
Availability: 7-10 days
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