UBE2T Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant UBE2T.
|Application ||WB, IP|
|Calculated MW||22521 Da|
|Other Names||Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T|
|Target/Specificity||UBE2T (AAH04152, 1 a.a. ~ 197 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||UBE2T Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).
1.Hypoxia disrupts the Fanconi anemia pathway and sensitizes cells to chemotherapy through regulation of UBE2T.Ramaekers CH, van den Beucken T, Meng A, Kassam S, Thoms J, Bristow RG, Wouters BG.Radiother Oncol. 2011 Jun 29. [Epub ahead of print]
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