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UBE4A Antibody (monoclonal) (M08)

Mouse monoclonal antibody raised against a partial recombinant UBE4A.

  • WB - UBE4A Antibody (monoclonal) (M08) AT4446a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - UBE4A Antibody (monoclonal) (M08) AT4446a
    UBE4A monoclonal antibody (M08), clone 1G8. Western Blot analysis of UBE4A expression in PC-12.
  • WB - UBE4A Antibody (monoclonal) (M08) AT4446a
    UBE4A monoclonal antibody (M08), clone 1G8. Western Blot analysis of UBE4A expression in NIH/3T3.
  • WB - UBE4A Antibody (monoclonal) (M08) AT4446a
    UBE4A monoclonal antibody (M08), clone 1G8. Western Blot analysis of UBE4A expression in K-562.
  • IF - UBE4A Antibody (monoclonal) (M08) AT4446a
    Immunofluorescence of monoclonal antibody to UBE4A on HeLa cell . [antibody concentration 10 ug/ml]
  • E - UBE4A Antibody (monoclonal) (M08) AT4446a
    Detection limit for recombinant GST tagged UBE4A is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14139
Other Accession NM_004788
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1G8
Calculated MW 122561 Da
Additional Information
Gene ID 9354
Other Names Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499)
Target/Specificity UBE4A (NP_004779, 974 a.a. ~ 1073 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUBE4A Antibody (monoclonal) (M08) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.


CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p53. Shi D, et al. Proc Natl Acad Sci U S A, 2009 Sep 22. PMID 19805293.Autoantibodies against ubiquitination factor E4A (UBE4A) are associated with severity of Crohn's disease. Sakiyama T, et al. Inflamm Bowel Dis, 2008 Mar. PMID 18069675.[Differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues] Yang Y, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2007 Jun. PMID 17553343.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation. Spinette S, et al. Cell Cycle, 2004 Dec. PMID 15611659.

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$ 350.00
Cat# AT4446a
Availability: 7-10 days
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