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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP14 Antibody (monoclonal) (M04)   

USP14 Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant USP14.

  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    USP14 monoclonal antibody (M04), clone 6D6 Western Blot analysis of USP14 expression in HeLa ( (Cat # AT4482a )
  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    USP14 monoclonal antibody (M04), clone 6D6. Western Blot analysis of USP14 expression in PC-12 ( (Cat # AT4482a )
  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    USP14 monoclonal antibody (M04), clone 6D6. Western Blot analysis of USP14 expression in Raw 264.7 ( (Cat # AT4482a )
  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    USP14 monoclonal antibody (M04), clone 6D6. Western Blot analysis of USP14 expression in NIH/3T3 ( (Cat # AT4482a )
  • WB - USP14 Antibody (monoclonal) (M04) AT4482a
    Western Blot analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody (M04), clone 6D6.

    Lane 1: USP14 transfected lysate(56.1 KDa).
    Lane 2: Non-transfected lysate.
  • IF - USP14 Antibody (monoclonal) (M04) AT4482a
    Immunofluorescence of monoclonal antibody to USP14 on HeLa cell. [antibody concentration 20 ug/ml]
  • E - USP14 Antibody (monoclonal) (M04) AT4482a
    Detection limit for recombinant GST tagged USP14 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54578
Other Accession NM_005151
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 6D6
Calculated MW 56069 Da
Additional Information
Gene ID 9097
Other Names Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14, USP14, TGT
Target/Specificity USP14 (NP_005142, 395 a.a. ~ 494 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUSP14 Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.


New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. Chen PC, et al. J Neurosci, 2009 Sep 2. PMID 19726649.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.USP14 inhibits ER-associated degradation via interaction with IRE1alpha. Nagai A, et al. Biochem Biophys Res Commun, 2009 Feb 20. PMID 19135427.Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation. Mines MA, et al. J Biol Chem, 2009 Feb 27. PMID 19106094.

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$ 350.00
Cat# AT4482a
Availability: 7-10 days
Bulk Size
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