DANRE atg4b Antibody (N-term)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|Application ||WB, E|
|Calculated MW||44453 Da|
|Antigen Region||1-30 aa|
|Other Names||Cysteine protease ATG4B, 3422-, Autophagy-related protein 4 homolog B, atg4b, apg4b|
|Target/Specificity||This DANRE atg4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DANRE atg4b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE). Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Cysteine protease required for autophagy, which is able to cleave the C-terminal part of proteins that may be subsequently converted to a smaller form, with a revealed C-terminal glycine, considered to be the phosphatidylethanolamine (PE)-conjugated form. This conjugated form has the capacity for the binding to autophagosomes (By similarity).
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