|Other Names||Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).|
|Cellular Location||Isoform Cytoplasmic: Cytoplasm, cytosol Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Note=Secretion is induced by TNF- alpha (PubMed:15851690). Cytosolic in quiescent mast cells Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739)|
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases are a class of enzymes thatcharge tRNAs with their cognate amino acids. Lysyl-tRNA synthetaseis a homodimer localized to the cytoplasm which belongs to theclass II family of tRNA synthetases. It has been shown to be atarget of autoantibodies in the human autoimmune diseases,polymyositis or dermatomyositis. Alternatively spliced transcriptvariants encoding different isoforms have been found for this gene.
McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)Segat, L., et al. Vaccine 28(10):2201-2206(2010)Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008)
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