|Other Names||Histone H2A type 2-A, Histone H2A2, Histone H2A/o, HIST2H2AA3, H2AFO, HIST2H2AA|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13700a was selected from the N-term region of HIST2H2AA3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bpof DNA is wrapped in repeating units, called nucleosomes. Thelinker histone, H1, interacts with linker DNA between nucleosomesand functions in the compaction of chromatin into higher orderstructures. This gene is intronless and encodes a member of thehistone H2A family. Transcripts from this gene lack polyA tails butinstead contain a palindromic termination element. This gene isfound in a histone cluster on chromosome 1. This gene is one offour histone genes in the cluster that are duplicated; this recordrepresents the centromeric copy.
Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006)Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)Boyne, M.T. II, et al. J. Proteome Res. 5(2):248-253(2006)Cao, R., et al. Mol. Cell 20(6):845-854(2005)Hagiwara, T., et al. Biochemistry 44(15):5827-5834(2005)
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