|Other Names||Cullin-4A, CUL-4A, CUL4A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13873a was selected from the N-term region of CUL4A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.|
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Provided below are standard protocols that you may find useful for product applications.
CUL4A is the ubiquitin ligase component of a multimericcomplex involved in the degradation of DNA damage-response proteins(Liu et al., 2009 [PubMed 19481525]).
Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010)Abbas, T., et al. Mol. Cell 40(1):9-21(2010)Lv, X.B., et al. J. Biol. Chem. 285(24):18234-18240(2010)Kerzendorfer, C., et al. Hum. Mol. Genet. 19(7):1324-1334(2010)Melchor, L., et al. Breast Cancer Res. 11 (6), R86 (2009) :
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