|Other Names||Tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, Kinase EMT, Kinase TLK, T-cell-specific kinase, Itk, Emt, Tlk, Tsk|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13916a was selected from the N-term region of Mouse Itk. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||Emt, Tlk, Tsk|
|Function||Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).|
|Cellular Location||Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.|
|Tissue Location||Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, heart, brain, intestine or testis. Expressed in T- lymphocytes and mast cells. It may also be expressed in natural killer cells|
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Provided below are standard protocols that you may find useful for product applications.
Itk may play a role in T-cell development, potentially in thymic selection.
Joseph, R.E., et al. J. Mol. Biol. 403(2):231-242(2010)Dierks, C., et al. Cancer Res. 70(15):6193-6204(2010)Grasis, J.A., et al. Mol. Cell. Biol. 30(14):3596-3609(2010)Xia, M., et al. J. Immunol. 184(12):6807-6814(2010)Sahu, N., et al. PLoS ONE 5 (6), E11348 (2010) :
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