|Other Names||Importin subunit alpha-5, Karyopherin subunit alpha-1, Nucleoprotein interactor 1, NPI-1, RAG cohort protein 2, SRP1-beta, Importin subunit alpha-5, N-terminally processed, KPNA1, RCH2|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed ubiquitously.|
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Recombination activating proteins RAG1 and RAG2 regulateand mediate V(D)J recombination, the process by which genes forimmunoglobulins and T-cell receptors are generated. Several otherubiquitously expressed proteins are thought to be recruited in therecombination process. Among these are the genes affected in severecombined immune deficiency and genes involved in ds-DNA breakrepair. The protein encoded by this gene interacts with RAG1 andmay play a role in V(D)J recombination. Two transcript variants,one protein-coding and the other not, have been found for thisgene.
O'Seaghdha, C.M., et al. Hum. Mol. Genet. 19(21):4296-4303(2010)Yang, S.N., et al. J. Biol. Chem. 285(26):19935-19946(2010)Bian, X.L., et al. Virus Res. 150 (1-2), 135-137 (2010) :Mateo, M., et al. J. Virol. 84(2):1169-1175(2010)Simkus, C., et al. Mol. Immunol. 46(7):1319-1325(2009)
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