|Other Names||Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3, 2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8636b was selected from the C-term region of human EHHADH. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.|
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Provided below are standard protocols that you may find useful for product applications.
EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.
Chen,G.L., et.al., Biochem. Biophys. Res. Commun. 178 (3), 1084-1091 (1991)Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)
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