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>   home   >   Products   >   Peptides   >   Blocking Peptides   >   DR4 Blocking Peptide    

DR4 Blocking Peptide

Product Information
Primary Accession O00220
Other Accession EAW63636.1
Gene ID 8797
Additional Information
Application & Usage The peptide is used for blocking the antibody activity of DR4. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD261, TNFRSF10A, APO2, DR4, TRAILR1
Target/Specificity DR4
Formulation 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsDR4 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms APO2, DR4, TRAILR1
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells EMBL; U90875; AAC51226.1; -; mRNA EMBL; BT006906; AAP35552.1; -; mRNA EMBL; AK291299; BAF83988.1; -; mRNA EMBL; EF064713; ABK41896.1; -; Genomic_DNA EMBL; AC100861; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC012866; AAH12866.1; -; mRNA CCDS; CCDS6039.1; - RefSeq; NP_003835.3; NM_003844.3 UniGene; Hs.213467; - UniGene; Hs.591834; - PDB; 5CIR; X-ray; 3.00 A; E/F/G=125-232 PDBsum; 5CIR; - ProteinModelPortal; O00220; - SMR; O00220; - BioGrid; 114325; 50 IntAct; O00220; 11 MINT; O00220; - STRING; 9606.ENSP00000221132; - BindingDB; O00220; - ChEMBL; CHEMBL3551; - GuidetoPHARMACOLOGY; 1879; - iPTMnet; O00220; - PhosphoSitePlus; O00220; - SwissPalm; O00220; - BioMuta; TNFRSF10A; - EPD; O00220; - MaxQB; O00220; - PaxDb; O00220; - PeptideAtlas; O00220; - PRIDE; O00220; - ProteomicsDB; 47790; - DNASU; 8797; - Ensembl; ENST00000221132; ENSP00000221132; ENSG00000104689 GeneID; 8797; - KEGG; hsa:8797; - UCSC; uc003xda.4; human CTD; 8797; - DisGeNET; 8797; - EuPathDB; HostDB:ENSG00000104689.9; - GeneCards; TNFRSF10A; - H-InvDB; HIX0022944; - HGNC; HGNC:11904; TNFRSF10A HPA; HPA050958; - HPA; HPA054475; - MIM; 603611; gene neXtProt; NX_O00220; - OpenTargets; ENSG00000104689; - PharmGKB; PA36597; - eggNOG; ENOG410IZX0; Eukaryota eggNOG; ENOG4111ZZM; LUCA GeneTree; ENSGT00730000110985; - HOGENOM; HOG000142423; - HOVERGEN; HBG061626; - InParanoid; O00220; - KO; K04722; - OMA; TACQCKP; - OrthoDB; EOG091G0MOV; - PhylomeDB; O00220; - TreeFam; TF333916; - Reactome; R-HSA-140534; Ligand-dependent caspase activation Reactome; R-HSA-202733; Cell surface interactions at the vascular wall Reactome; R-HSA-3371378; Regulation by c-FLIP Reactome; R-HSA-5213460; RIPK1-mediated regulated necrosis Reactome; R-HSA-5218900; CASP8 activity is inhibited Reactome; R-HSA-6803211; TP53 Regulates Transcription of Death Receptors and Ligands Reactome; R-HSA-69416; Dimerization of procaspase-8 Reactome; R-HSA-75158; TRAIL signaling SignaLink; O00220; - SIGNOR; O00220; - GeneWiki; TNFRSF10A; - GenomeRNAi; 8797; - PRO; PR:O00220; - Proteomes; UP000005640; Chromosome 8 Bgee; ENSG00000104689; - CleanEx; HS_TNFRSF10A; - ExpressionAtlas; O00220; baseline and differential Genevisible; O00220; HS GO; GO:0009986; C:cell surface; IDA:UniProtKB GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central GO; GO:0005622; C:intracellular; IEA:GOC GO; GO:0005886; C:plasma membrane; TAS:Reactome GO; GO:0005035; F:death receptor activity; TAS:ProtInc GO; GO:0002020; F:protease binding; IPI:UniProtKB GO; GO:0038023; F:signaling receptor activity; NAS:UniProtKB GO; GO:0045569; F:TRAIL binding; NAS:UniProtKB GO; GO:0008134; F:transcription factor binding; IPI:UniProtKB GO; GO:0005031; F:tumor necrosis factor-activated receptor activity; IBA:GO_Central GO; GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; TAS:Reactome GO; GO:0007250; P:activation of NF-kappaB-inducing kinase activity; NAS:UniProtKB GO; GO:0006915; P:apoptotic process; NAS:UniProtKB GO; GO:0007166; P:cell surface receptor signaling pathway; TAS:Reactome GO; GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB GO; GO:0097191; P:extrinsic apoptotic signaling pathway; IDA:UniProtKB GO; GO:0008625; P:extrinsic apoptotic signaling pathway via death domain receptors; TAS:ProtInc GO; GO:0006955; P:immune response; IBA:GO_Central GO; GO:0006954; P:inflammatory response; IBA:GO_Central GO; GO:0050900; P:leukocyte migration; TAS:Reactome GO; GO:0007275; P:multicellular organism development; IBA:GO_Central GO; GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IBA:GO_Central GO; GO:1902042; P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; TAS:Reactome GO; GO:0042981; P:regulation of apoptotic process; TAS:Reactome GO; GO:0042127; P:regulation of cell proliferation; IBA:GO_Central GO; GO:1902041; P:regulation of extrinsic apoptotic signaling pathway via death domain receptors; TAS:Reactome GO; GO:0032496; P:response to lipopolysaccharide; IBA:GO_Central GO; GO:0007165; P:signal transduction; TAS:ProtInc GO; GO:0036462; P:TRAIL-activated apoptotic signaling pathway; IDA:ParkinsonsUK-UCL CDD; cd08315; Death_TRAILR_DR4_DR5; 1 CDD; cd10580; TNFRSF10; 1 InterPro; IPR011029; DEATH-like_dom_sf InterPro; IPR000488; Death_domain InterPro; IPR001368; TNFR/NGFR_Cys_rich_reg InterPro; IPR020465; TNFR_10 InterPro; IPR034024; TNFRSF10_N InterPro; IPR034029; TNFRSF10A/B_death Pfam; PF00531; Death; 1 Pfam; PF00020; TNFR_c6; 2 PIRSF; PIRSF037867; CD261_antigen; 1 PRINTS; PR01956; TNFACTORR10 SMART; SM00005; DEATH; 1 SMART; SM00208; TNFR; 2 SUPFAM; SSF47986; SSF47986; 1 PROSITE; PS50017; DEATH_DOMAIN; 1 PROSITE; PS00652; TNFR_NGFR_1; 2 PROSITE; PS50050; TNFR_NGFR_2; 2 1: Evidence at protein level; 3D-structure; Apoptosis; Complete proteome; Disulfide bond; Glycoprotein; Membrane; Methylation; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix SIGNAL 1 23 CHAIN 24 468 Tumor necrosis factor receptor superfamily member 10A /FTId=PRO_0000034579 TOPO_DOM 24 239 Extracellular. TRANSMEM 240 262 Helical. TOPO_DOM 263 468 Cytoplasmic. REPEAT 107 145 TNFR-Cys 1 REPEAT 147 188 TNFR-Cys 2 REPEAT 189 229 TNFR-Cys 3 DOMAIN 365 448 Death. {ECO:0000255|PROSITE- ProRule:PRU00064} COMPBIAS 29 32 Poly-Ala MOD_RES 52 52 Omega-N-methylarginine MOD_RES 424 424 Phosphoserine MOD_RES 463 463 Phosphoserine MOD_RES 466 466 Phosphoserine CARBOHYD 156 156 N-linked (GlcNAc...) asparagine DISULFID 132 145 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 148 164 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 167 180 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 170 188 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 190 204 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 207 221 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} DISULFID 211 229 {ECO:0000244|PDB:5CIR, ECO:0000255|PROSITE-ProRule:PRU00206, ECO:0000269|PubMed:26457518} VARIANT 11 11 G -> V (in dbSNP:rs34737614) /FTId=VAR_052349 VARIANT 33 33 T -> I (in dbSNP:rs20577) /FTId=VAR_016149 VARIANT 105 105 P -> R (in dbSNP:rs11986840) /FTId=VAR_052350 VARIANT 141 141 H -> R (in dbSNP:rs17620) /FTId=VAR_016150 VARIANT 209 209 R -> T (in dbSNP:rs20575) /FTId=VAR_016151 VARIANT 228 228 E -> A (in dbSNP:rs20576) /FTId=VAR_016152 VARIANT 297 297 N -> H (in dbSNP:rs17088980) /FTId=VAR_052351 VARIANT 441 441 R -> K (in dbSNP:rs2230229) {ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:9082980, ECO:0000269|Ref.2, ECO:0000269|Ref.4} /FTId=VAR_052352 CONFLICT 281 281 W -> C (in Ref. 3; BAF83988) STRAND 136 138 {ECO:0000244|PDB:5CIR} STRAND 145 147 {ECO:0000244|PDB:5CIR} TURN 150 152 {ECO:0000244|PDB:5CIR} STRAND 157 159 {ECO:0000244|PDB:5CIR} STRAND 174 178 {ECO:0000244|PDB:5CIR} STRAND 187 190 {ECO:0000244|PDB:5CIR} STRAND 216 219 {ECO:0000244|PDB:5CIR} STRAND 228 230 {ECO:0000244|PDB:5CIR} SEQUENCE 468 AA; 50089 MW; 7E96619D0BDC0CD4 CRC64; MAPPPARVHL GAFLAVTPNP GSAASGTEAA AATPSKVWGS SAGRIEPRGG GRGALPTSMG QHGPSARARA GRAPGPRPAR EASPRLRVHK TFKFVVVGVL LQVVPSSAAT IKLHDQSIGT QQWEHSPLGE LCPPGSHRSE HPGACNRCTE GVGYTNASNN LFACLPCTAC KSDEEERSPC TTTRNTACQC KPGTFRNDNS AEMCRKCSRG CPRGMVKVKD CTPWSDIECV HKESGNGHNI WVILVVTLVV PLLLVAVLIV CCCIGSGCGG DPKCMDRVCF WRLGLLRGPG AEDNAHNEIL SNADSLSTFV SEQQMESQEP ADLTGVTVQS PGEAQCLLGP AEAEGSQRRR LLVPANGADP TETLMLFFDK FANIVPFDSW DQLMRQLDLT KNEIDVVRAG TAGPGDALYA MLMKWVNKTG RNASIHTLLD ALERMEERHA REKIQDLLVD SGKFIYLEDG TGSAVSLE
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Cat# PBV10025b-50
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