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>   home   >   Products   >   Peptides   >   Blocking Peptides   >   Oct-4 Blocking Peptide   

Oct-4 Blocking Peptide

Product Information
Primary Accession P20263
Gene ID 18999
Calculated MW 38216 Da
Additional Information
Gene ID 18999
Application & Usage The peptide is used for blocking the antibody activity of Oct-4. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3
Target/Specificity 40455
Formulation 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsOct-4 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pou5f1
Synonyms Oct-3, Oct-4, Otf-3, Otf3
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q01860}. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163, ECO:0000269|PubMed:29153991} Note=Expressed in a diffuse and slightly punctuate pattern (By similarity). Colocalizes with MAPK8 and MAPK9 in the nucleus (PubMed:29153991). {ECO:0000250|UniProtKB:Q01860, ECO:0000269|PubMed:29153991}
Tissue Location Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues
Research Areas
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$ 95.00
Cat# PBV10242b-50
Availability: 5-7days
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