ELITE™ Polyclonal Antibody Services
Abgent is a leading manufacturer of off the shelf and custom antibodies with over 20,000 antibodies developed over the last decade. Our polyclonal antibody services provide a fast and economical solution with extreme flexibility - we offer primary Polyclonal Antibody Service (see list below ) to choose from, where you can provide your own immunogen or work with our world-class synthetic peptide chemistry group to design an epitope-specific and immunogenic peptide to meet your specifications.
Additionally, Abgent can develop a pre-optimized antibody validated in the assay of your choosing. Please contact us to learn more about these and other service offerings.
Our ELITE™ polyclonal antibody service offering list includes the following:
Peptide antigen design and preparation: Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 80%
Peptide conjugation to KLH
|Protein antigen design: Recombinant protein preparation||7-14|
One primary subcutaneous injection of 200 ug conjugated peptide/protein in Complete
Five subcutaneous injections of 100 ug conjugated peptide/protein in Incomplete Freund’s
|Test bleeds and ELISA analysis on test bleed #1, and test bleed #2 if #1 is negative||35-49|
|Five production bleeds||39-69|
|Terminal bleed by exsanguination (±40 ml serum/rabbit), final ELISA analysis||69|
|Peptide affinity purification for total yield of 2-10 mg IgG from 2 rabbits||76|
|Validation test by WB (optional and limited guarantee available)||3|
|Validation test by IF (optional and limited guarantee available)||3|
|Validation by IHC (optional and limited guarantee available)||3|
|Validation by FC (optional and limited guarantee available)||3|
In order to minimise your expense Abgent offers customised package (see example below):
ELITE™ Polyclonal Antibody Services Premier Package
• Peptide sequence • ELISA titer • 1ml of prebleed per rabbit • Post immunization bleeds as requested
Project Timeline: 12-14 weeks
How It Works:
Customers can simply select their desired services or the whole polyclonal antibody package and send us your specific requirements.
Our experienced tech support representatives can also guide you in the process of selecting the service package that best suits your research goals.
Abgent will deliver your polyclonal antibody with guaranteed quality in timely manner.