Biotinylated Anti-Human Adipolean Variant Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAdipolean Variant. Anti-Human Adipolean Variant specific antibody was purified by affinity chromatography and then biotinylated. |
---|---|
WesternBlot | To detect hAdipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAdipolean is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect hAdipolean Variant by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human Adipolean Variant ( ) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hAdipolean Variant. |
Direct | To detect hAdipolean Variant by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hAdipolean Variant. |
Storage | -20°C |
Precautions | Biotinylated Anti-Human Adipolean Variant Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.