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Anti-Human IGF-BP1 Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Anti-Human IGF-BP1 specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP1 matrix.
WesternBlot To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect hIGF-BP1 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IGF-BP1 (60-188BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta.  The recommended concentration is 0.125 µg/mL- 0.200 µg/mL with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.    Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. 
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human IGF-BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10174
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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