|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT . Anti-Human LIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix.|
|WesternBlot||To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect hLIGHT by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human LIGHT (60-206BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 2.5 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 buffer is recommended. Optimal concentrations and conditions may vary. |
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human LIGHT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.