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Anti-Human MEC Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Anti-Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix.
WesternBlot To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect hMEC by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MEC (60-218BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.
Immunohistochemistry This antibody stained CACO-2 cells.  The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.

*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human MEC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10385
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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