|Application ||WB, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA-2 (human MIA-2). Anti-Human MIA-2 specific antibody was purified by affinity chromatography and then biotinylated.|
|WesternBlot||To detect Human MIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human MIA-2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Human MIA-2 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti- Human MIA-2 (60-220P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human MIA-2.|
|Direct||To detect Human MIA-2 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human MIA-2.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Biotinylated Anti-Human MIA-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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