|Application ||WB, IHC, E|
|Preparation||Produced in BALB/c mice using highly pure recombinant Human MIP-3β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.|
|WesternBlot||To detect Human MIP-3β by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3β is 2.0-4.0 ng/lane, under reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MIP-3β when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3β (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.|
|Immunohistochemistry||This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human MIP-3β Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.