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Anti-Human Resistin Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Additional Information
Preparation Produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen. This IgG2bκ antibody was purified from cell culture by Protein A affinity chromatography.
WesternBlot To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
Sandwich In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with BioGems' biotinylated antigen affinity purified anti-human Resistin (60-289BT) as the detection antibody at a concentration of at least 0.5-1.0 µg/ml.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 μg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Formulation A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human Resistin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 216.00
Cat# ABG10484
(40 western blots)
Availability: 5-7days
Bulk Size
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