|Application ||WB, IHC, E|
|Preparation||Produced in mice using highly pure recombinant Human soluble IL-2 Receptor α as the immunizing antigen. This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.|
|WesternBlot||To detect Human sIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Rα is 2.0-4.0 ng/lane, under non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Rα when used in conjunction with compatible secondary reagents.|
|Immunohistochemistry||This antibody stained frozen human tonsil. The recommended concentration is 5.0 μg/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human sIL-2Rα Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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