|Application ||WB, IHC, E|
|Preparation||Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human TRAIL/APO2L as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.|
|WesternBlot||To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human sTRAIL/APO2L when used with BioGems' biotinylated antigen affinity purified anti-human sTRAIL/APO2L (60-330BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 µg/mL- 1.5 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary. |
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human TRAIL/Apo2L (10.0 ng/ml), a concentration of 0.30-0.60 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human sTRAIL/Apo2L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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