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Anti-Murine VEGF Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF. Anti-Murine VEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.
WesternBlot To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine VEGF (61-127BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5μg/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
Neutralization To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (10.00 ng/ml), a concentration of 0.08-0.12 µg/ml of this antibody is required.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Murine VEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10577
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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