|Application ||WB, E|
|Preparation||Produced in BALB/c mice using highly pure (>98%) recombinant human VEGF165 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A chromatography.|
|WesternBlot||To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with BioGems' biotinylated antigen affinity purified anti-human VEGF165 (60-342BT) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of ≤ 0.2 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human VEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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