|Application ||WB, E|
|Preparation||Produced in BALB/c mice using highly pure (>98%) recombinant human VEGF165 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A chromatography.|
|WesternBlot||To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with BioGems' biotinylated antigen affinity purified anti-human VEGF165 (60-342BT) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of ≤ 0.2 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human VEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.