|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for 14-3-3 sigma detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1|
|Calculated MW||27774 Da|
|Application Details||Western blot, 0.1-0.5 µg/ml|
Direct ELISA, 0.1-0.5 µg/ml
|Subcellular Localization||Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway.|
|Tissue Specificity||Present mainly in tissues enriched in stratified squamous keratinizing epithelium.|
|Contents||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C; for one year. After r˚Constitution, at 4˚C; for one month. It˚Can also be aliquotted and stored frozen at -20˚C; for a longer time. Avoid repeated freezing and thawing.|
|Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.|
|Cellular Location||Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway|
|Tissue Location||Present mainly in tissues enriched in stratified squamous keratinizing epithelium|
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Provided below are standard protocols that you may find useful for product applications.
Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
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