|Application ||IHC-P, FC, E|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for TAPA1 detection. Tested with IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81, CD81, TAPA1, TSPAN28|
|Calculated MW||25809 Da|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml|
Flow Cytometry, 1-3Î¼g/1x106 cells
Direct ELISA, 0.1-0.5 µg/ml
|Subcellular Localization||Basolateral cell membrane.|
|Tissue Specificity||Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.|
|Contents||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived human TAPA1 recombinant protein (Position: F113-K201).|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C; for one year. After r˚Constitution, at 4˚C; for one month. It˚Can also be aliquotted and stored frozen at -20˚C; for a longer time. Avoid repeated freezing and thawing.|
|Function||May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein. Note=Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane|
|Tissue Location||Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines|
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Provided below are standard protocols that you may find useful for product applications.
CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
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