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Anti-ALPP Picoband Antibody

     
  • WB - Anti-ALPP Picoband Antibody ABO10209
    Western blot analysis of ALPP expression in human placenta extract (lane 1). ALPP at 70KD was detected using rabbit anti- ALPP Antigen Affinity purified polyclonal antibody (Catalog # ABO10209) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-ALPP Picoband Antibody ABO10209
    ALPP was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- ALPP Antigen Affinity purified polyclonal antibody (Catalog # ABO10209) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P05187
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Alkaline phosphatase, placental type(ALPP) detection. Tested with WB, IHC-P in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 250
Other Names Alkaline phosphatase, placental type, 3.1.3.1, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP
Calculated MW 57954 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity Detected in placenta (at protein level). .
Protein Name Alkaline phosphatase, placental type
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ALPP (403-433aa KARDRKAYTVLLYGNGPGYVLKDGARPDVTE).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name ALPP
Synonyms PLAP
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Detected in placenta (at protein level).
Research Areas
Citations (0)

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Background

Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

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$ 280.00
Cat# ABO10209
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