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Anti-CKAP4 Picoband Antibody

     
  • WB - Anti-CKAP4 Picoband Antibody ABO10232
    Western blot analysis of CKAP4 expression in mouse brain extract. CKAP4 at 66KD was detected using rabbit anti- CKAP4 Antigen Affinity purified polyclonal antibody (Catalog # ABO10232) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8BMK4
Host Rabbit
Reactivity Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Cytoskeleton-associated protein 4(Ckap4) detection. Tested with WB in Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 216197
Other Names Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, Ckap4
Calculated MW 63692 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Translocates to the perinuclear region upon APF- stimulation. .
Protein Name Cytoskeleton-associated protein 4
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse CKAP4 (95-118aa LVAAAAFSGWYVHHVLEEVQQVRR), different from the related human sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Ckap4
Function High-affinity epithelial cell surface receptor for APF.
Cellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note=Translocates to the perinuclear region upon APF- stimulation.
Research Areas
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Background

Cytoskeleton-associated protein 4, also known as CLIMP63 and p63, is a protein that in humans is encoded by the CKAP4 gene. It is mapped to 12q23.3. CKAP4 is found on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. It has a bimodal distribution. First, CKAP4 is found on the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels). Second, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent on its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER.

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$ 240.00
Cat# ABO10232
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