Anti-Sca1/Ly6A/E Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P05533 |
Host | Rabbit |
Reactivity | Mouse |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Lymphocyte antigen 6A-2/6E-1(Ly6a) detection. Tested with WB in Mouse. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 110454 |
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Other Names | Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly6a, Ly6 |
Calculated MW | 14377 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Mouse |
Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
Tissue Specificity | Widely expressed. . |
Protein Name | Lymphocyte antigen 6A-2/6E-1 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL). |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins. |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | Ly6a |
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Synonyms | Ly6 |
Function | T-cell activation. |
Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor. |
Tissue Location | Widely expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.
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