|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for alpha-Tubulin detection. Tested with WB, IHC-P in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, Detyrosinated tubulin alpha-1A chain, TUBA1A, TUBA3|
|Calculated MW||50136 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, mouse, rat, chicken, By Heat|
Western blot, 2 µg/ml, Human, mouse, rat, chicken
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Tissue Specificity||Expressed at a high level in fetal brain. .|
|Protein Name||Tubulin alpha-1A chain|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Microtubules from chicken embryo brain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.|
|Cellular Location||Cytoplasm, cytoskeleton.|
|Tissue Location||Expressed at a high level in fetal brain.|
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Provided below are standard protocols that you may find useful for product applications.
Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
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