Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, ICC |
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Primary Accession | D3ZHG8 |
Host | Mouse |
Isotype | Mouse IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1; GGA2; GGA3 ) detection. Tested with WB, ICC in Human,mouse,rat. No cross reactivity with other proteins. |
Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Calculated MW | 71715 MW KDa |
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Application Details | Immunocytochemistry , 1 µg/ml µg/ml, Human, mouse, rat, - Western blot, 0.25-0.5 µg/ml µg/ml, Human, mouse, rat |
Subcellular Localization | GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane;Peripheral membrane protein.|Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA2: Golgi apparatus, trans-Golgi networkmembrane |Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA3: Golgi apparatus, trans-Golgi networkmembrane |Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |
Tissue Specificity | GGA1: Ubiquitously expressed. |
Protein Name | ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3 |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
Clone Names | GTU-88 |
Immunogen | Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH. |
Purification | Ascites |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the GGA protein family. |
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Background
Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
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