|Application ||WB, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1; GGA2; GGA3 ) detection. Tested with WB, ICC in Human,mouse,rat. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Calculated MW||71715 MW KDa|
|Application Details||Immunocytochemistry , 1 µg/ml µg/ml, Human, mouse, rat, -|
Western blot, 0.25-0.5 µg/ml µg/ml, Human, mouse, rat
|Subcellular Localization||GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane;Peripheral membrane protein.|Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA2: Golgi apparatus, trans-Golgi networkmembrane |Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA3: Golgi apparatus, trans-Golgi networkmembrane |Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane|
|Tissue Specificity||GGA1: Ubiquitously expressed.|
|Protein Name||ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the GGA protein family.|
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Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
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