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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Lamin B1 Antibody   

Anti-Lamin B1 Antibody

     
  • WB - Anti-Lamin B1 Antibody ABO10534
    Anti-Lamin B1 antibody, ABO10534, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell Lysate
  • IHC - Anti-Lamin B1 Antibody ABO10534
    Anti-Lamin B1 antibody, ABO10534, IHC(P)IHC(P): Human Mammary Cancer Tissue
  • IHC - Anti-Lamin B1 Antibody ABO10534
    Anti-Lamin B1 antibody, ABO10534, IHC(F)IHC(F): Rat Intestine Tissue
  • ICC - Anti-Lamin B1 Antibody ABO10534
    Anti-Lamin B1 antibody, ABO10534, ICCICC: A549 Cell
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P20700
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4001
Other Names Lamin-B1, LMNB1, LMN2, LMNB
Calculated MW 66408 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat

Immunocytochemistry , 0.5-1 µg/ml, Human, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Protein Name Lamin-B1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the intermediate filament family.
Protein Information
Name LMNB1
Synonyms LMN2, LMNB
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Cellular Location Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Research Areas
Citations (0)

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Background

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

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$ 280.00
Cat# ABO10534
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