Anti-CD86/B7-2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P42082 |
Host | Rabbit |
Reactivity | Mouse |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD86(CD86) detection. Tested with WB in Mouse. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 12524 |
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Other Names | T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86 |
Calculated MW | 34666 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Mouse |
Subcellular Localization | Cell membrane ; Single-pass type I membrane protein . |
Tissue Specificity | Expressed on activated B-cells. |
Protein Name | T-lymphocyte activation antigen CD86 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD86(66-80aa YEHYLGTEKLDSVNA), different from the related rat sequence by two amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Name | Cd86 |
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Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883). |
Cellular Location | Cell membrane; Single-pass type I membrane protein |
Tissue Location | Expressed on activated B-cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD86 is officially called Cluster of Differentiation 86, also known as B7-2. Being a member of the immunoglobulin superfamily, it is a protein expressed on antigen-presenting cells. The CD86 gene is mapped to human chromosome 3q21. The smallest transcript, 828 bp, which the authors termed CD86deltaTM, has a deletion from nucleotide 686 to nucleotide 829(i.e., exon 6) and encodes a 275-amino acid protein. CD86deltaTM enhances proliferation and cytokine production by both naive and memory T cells, providing costimulatory signals necessary for T cell activation and survival.
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