|Application ||WB, ICC|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 1(EIF2AK1) detection. Tested with WB, ICC in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Eukaryotic translation initiation factor 2-alpha kinase 1, 188.8.131.52, Heme-controlled repressor, HCR, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2-alpha kinase, EIF2AK1, HRI, KIAA1369|
|Calculated MW||71106 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human, -|
Immunocytochemistry , 0.5-1 µg/ml, Human
|Subcellular Localization||Cytoplasm .|
|Tissue Specificity||Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). .|
|Protein Name||Eukaryotic translation initiation factor 2-alpha kinase 1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human EIF2AK1(466-483aa CTDILQKNTDWTNRNGKR).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.|
|Function||Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).|
|Tissue Location||Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
EIF2AK1(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1), also called HRI, is an enzyme that in humans is encoded by the EIF2AK1 gene. Hartz(2010) mapped the EIF2AK1 gene to chromosome 7p22.1 based on an alignment of the EIF2AK1 sequence with the genomic sequence. Chen and London(1995) reviewed the activation and control of rat and rabbit Hri. Hri functions as a dimer, and its activation involves autophosphorylation, which requires an invariant lysine in kinase domain II. Using cDNA arrays and Northern blot analysis, Hwang et al.(2000) found that HRI was downregulated in the majority of ovarian cancers examined compared with normal ovarian tissues.
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