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Anti-EIF2AK1 Antibody

     
  • WB - Anti-EIF2AK1 Antibody ABO11331
    Anti-EIF2AK1 antibody, ABO11331, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK1, 35.5KD(162aa tag+ R398-K551)Lane 1: Recombinant Human EIF2AK1 Protein 10ngLane 2: Recombinant Human EIF2AK1 Protein 5ngLane 3: Recombinant Human EIF2AK1 Protein 2.5ng
  • ICC - Anti-EIF2AK1 Antibody ABO11331
    Anti-EIF2AK1 antibody, ABO11331, ICCICC: HELA Cell
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q9BQI3
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 1(EIF2AK1) detection. Tested with WB, ICC in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 27102
Other Names Eukaryotic translation initiation factor 2-alpha kinase 1, 2.7.11.1, Heme-controlled repressor, HCR, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2-alpha kinase, EIF2AK1, HRI, KIAA1369
Calculated MW 71106 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, -
Immunocytochemistry , 0.5-1 µg/ml, Human
Subcellular Localization Cytoplasm .
Tissue Specificity Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). .
Protein Name Eukaryotic translation initiation factor 2-alpha kinase 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human EIF2AK1(466-483aa CTDILQKNTDWTNRNGKR).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Protein Information
Name EIF2AK1
Synonyms HRI, KIAA1369
Function Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).
Cellular Location Cytoplasm.
Tissue Location Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level)
Research Areas
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Background

EIF2AK1(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1), also called HRI, is an enzyme that in humans is encoded by the EIF2AK1 gene. Hartz(2010) mapped the EIF2AK1 gene to chromosome 7p22.1 based on an alignment of the EIF2AK1 sequence with the genomic sequence. Chen and London(1995) reviewed the activation and control of rat and rabbit Hri. Hri functions as a dimer, and its activation involves autophosphorylation, which requires an invariant lysine in kinase domain II. Using cDNA arrays and Northern blot analysis, Hwang et al.(2000) found that HRI was downregulated in the majority of ovarian cancers examined compared with normal ovarian tissues.

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$ 240.00
Cat# ABO11331
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