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Anti-MBD4 Antibody

     
  • WB - Anti-MBD4 Antibody ABO11350
    Anti-MBD4 antibody, ABO11350, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD(162aa tag+ Q400-S580)Lane 1: Recombinant Human MBD4 Protein 10ngLane 2: Recombinant Human MBD4 Protein 5ngLane 3: Recombinant Human MBD4 Protein 2.5ng
  • WB - Anti-MBD4 Antibody ABO11350
    Anti-MBD4 antibody, ABO11350, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: MCF-7 Cell Lysate
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O95243
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 4(MBD4) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 8930
Other Names Methyl-CpG-binding domain protein 4, 3.2.2.-, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1
Calculated MW 66051 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Nucleus.
Protein Name Methyl-CpG-binding domain protein 4
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4(566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 1 MBD (methyl-CpG-binding) domain.
Protein Information
Name MBD4
Synonyms MED1
Function Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Cellular Location Nucleus.
Citations (0)

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Background

MBD4(Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

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$ 240.00
Cat# ABO11350
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