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Anti-Periostin/OSF2 Antibody

     
  • WB - Anti-Periostin/OSF2 Antibody ABO11355
    Anti- Periostin antibody, ABO11355, Western blottingAll lanes: Anti Periostin (ABO11355) at 0.5ug/mlWB: Mouse Cardiac Muscle Tissue Lysate at 50ugPredicted bind size: 93KDObserved bind size: 93KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q62009
Host Rabbit
Reactivity Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Periostin(POSTN) detection. Tested with WB in Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 50706
Other Names Periostin, PN, Osteoblast-specific factor 2, OSF-2, Postn, Osf2
Calculated MW 93144 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Mouse, Rat
Subcellular Localization Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.
Tissue Specificity Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .
Protein Name Periostin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin(24-41aa NSYYDKVLAHSRIRGRDQ), identical to the related rat sequence, and different from the related human sequence by three amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 1 EMI domain.
Protein Information
Name Postn
Synonyms Osf2
Function Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:10404027). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (PubMed:20181949).
Cellular Location Golgi apparatus. Secreted Secreted, extracellular space, extracellular matrix. Note=Colocalizes with BMP1 in the Golgi (PubMed:20181949).
Tissue Location Preferentially expressed in periosteum and periodontal ligament (PubMed:10404027). Also expressed in the developing and adult heart (PubMed:11335131)
Research Areas
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Background

POSTN(Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Gillan et al.(2002) found that purified recombinant PN supported adhesion of ovarian epithelial cells. Shao et al.(2004) found that periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. Kuhn et al.(2007) showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

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$ 240.00
Cat# ABO11355
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